ANALYSIS OF CHANGES IN HUMORAL IMMUNITY INDICATORS AFTER STANDARD THERAPY IN CHRONIC GENERALIZED PERIODONTITIS FOR PATIENTS WITH TYPE II DIABETES MELLITUS

Abstract

Subject. The subject of the study is indicators of humoral immunity after standard therapy for chronic generalized periodontitis in patients with type II diabetes mellitus. Objectives. The goal is to assess the levels of cytokines and secretory IgA in the gingival fluid of patients before and after standard treatment of chronic generalized periodontitis: interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), tumor necrosis factor beta (TNF-β), interleukin 12 (IL-12), and its subtypes P 40 and P 70, interleukin 17 (IL-17), interferon gamma (IFN-γ), interleukin 10 (IL-10), interleukin 4 (IL-4), interleukin 13 (IL-13), transforming growth factor beta 1 (TGF-β1) and secretory IgA (sIgA) in patients with chronic generalized periodontitis without concomitant pathology (group I) and with chronic generalized periodontitis and diabetes mellitus Type II (group II). Methodology. 96 patients were examined, of which: 47 people – group I, 49 people – group II. The control group consisted of healthy volunteers (30 people). The levels of the studied cytokines were determined by the sandwich version of enzyme-linked immunosorbent assay using specific reagents “R&D Diagnostics Inc” (USA) and sIgA – “IgA secretory-ELISA-BEST” (Russia). Results. In patients of all study groups with mild periodontitis, normalization of the levels of cytokines and secretory IgA was established after standard treatment. In groups of patients with moderate and severe severity of chronic periodontitis, persistence of mucosal immune dysfunction was recorded. Conclusions. The data obtained indicate the need to prescribe immunotropic therapy in patients with moderate and severe severity of chronic generalized periodontitis.