Hepatocyte Nuclear Factor -1α stimulates cervical cancer cells to migrate and invade through regulating pyruvate kinase L/R-Reference-Cited by-同舟云学术

Hepatocyte Nuclear Factor -1α stimulates cervical cancer cells to migrate and invade through regulating pyruvate kinase L/R

Published:2021-09-02 Issue:3 Volume:62 Page:236-246
ISSN:2477-9393
Container-title:Investigación Clínica
language:
Short-container-title:Invest Clin

Author:

Tao Xiao-Ling¹,Yu Wei-Chang¹,Chen De-Jun¹,Wang Li-Ming¹,Liu Lu¹,Xing Qi²

Affiliation:

1. Department of Gynecology, Hubei Provincial Maternal and Child Health Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

2. Department of Neurological Rehabilitation, Hubei Provincial Maternal and Child Health Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

Abstract

This study was aimed to analyze the role of hepatocyte nuclear factor -1α (HNF-1α) in regulating migrative and invasive potentials in cervical cancer via the involvement of pyruvate kinase L/R (PKLR). The expression of HNF-1α and PKLR in cervical cancer tissues classified by tumor size and FIGO (Federation International of Gynecology and Obstetrics) stage were detected by qRT-PCR. The expression correlation between HNF-1α and PKLR in cervical cancer tissues was analyzed by Pearson correlation test. After intervening HNF-1α and PKLR levels in SiHa and Hela cells, their migratory and invasive abilities were examined by the Transwell assay. HNF-1α was upregulated in cervical cancer tissues, particularly those with large tumor size or advanced FIGO stage. PKLR was highly expressed in cervical cancer tissues as well, presenting a positive correlation with the HNF-1α level. Knockdown of HNF-1α attenuated migratory and invasive abilities in SiHa cells, whereas overexpression of HNF-1α enhanced migratory and invasive abilities in SiHa cells. PKLR was able to abolish the regulatory effects of HNF-1α on cervical cancer metastasis. HNF-1α and PKLR synergistically promote cervical cancer to migrate and invade.

Publisher

Universidad del Zulia

Subject

General Medicine

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