Genetic Diversity of Mulberry Cultivars using ISSR and SRAP Molecular Markers

Abstract

Mulberry, a kind of Morus (Moraceae), is an important perennial economic plant, with leaves used to feed silkworm. It is important to reveal the relationship between the genetic variation and evolution of mulberry and to reveal the phylogenetic of its germplasm. In the present study, the genetic diversity of the gene structure characteristics of 23 main mulberry in Sichuan Province (China) based on inter-simple sequence related (ISSR), sequence-related amplified polymorphism (SRAP) markers and sequencing analysis was reported. 12 ISSRs and 5 SRAPs were exploited, chosen to evaluate the polymorphism level and the discriminating capacity from 100 ISSR primers and 50 SRAP primer. The 12 ISSRs generated 116 repeatable amplified bands, of which 93 (80.24%) were polymorphic. The amplified fragment size was between 0.1–1.5 KB. The 5 SRAP primers produced 31 repeatable amplified bands, of which 24 (77.42%) were polymorphic. The amplified fragment size was also between 0.1–1.5 KB. The study indicated that, ISSR and SRAP markers were reliable and effective to assess the genetic relationship and genetic diversity of 23 main mulberry varieties of Sichuan Province (China). The results provided the scientific basis for the establishment and identification of DNA fingerprint of mulberry varieties germplasm resources. © 2019 Friends Science Publishers