Genetic monitoring of sterlet juveniles (Acipenser ruthenus) in the Volga river-Reference-Cited by-同舟云学术

Genetic monitoring of sterlet juveniles (Acipenser ruthenus) in the Volga river

Published:2023-05-16 Issue:5 Volume: Page:305-315
ISSN:2074-5990
Container-title:Rybovodstvo i rybnoe hozjajstvo (Fish Breeding and Fisheries)
language:
Short-container-title:Fish Breeding and Fisheries

Author:

Makarova E.G.^ORCID, ,Kozlova N.V.^ORCID,

Abstract

The aim of the work was to carry out genetic monitoring of the natural reproduction of juveniles (larvae and underyearlings) of sterlet (Acipenser ruthenus Linnaeus, 1958), caught in the middle and lower spawning zones of the Volga River for the period 2021–2022, which is necessary and relevant in the context of a decrease in the number of sturgeon species of fish. The study of fish was carried out according to five nuclear markers and a fragment of the control region of mitochondrial DNA.According to the results of microsatellite analysis, sterlet individuals were characterized by species-specific dominant alleles and high values of the main indicators of genetic diversity: the number of alleles, allelic diversity and heterozygosity, and a slight deficit of heterozygotes was noted. By analyzing of the nucleotide sequences of the mtDNA D-loop region a high level of diversity of maternally inherited haplotypes was revealed, reflecting the number of females involved in natural reproduction. According to the monitoring data, no differences in the distribution and number of identified variants of the D-loop region were registered in the interannual aspect. During the study period, 48 sterlet mitochondrial haplotypes were recorded, of which 18 mtDNA variations were unique, occurring in the Volga-Caspian fishery basin. In the study of sterlet larvae by molecular genetic methods in the Volga River was revealed the process of hybridization between stellate sturgeon and sterlet (Acipenser stellatus × Acipenser ruthenus).

Publisher

PANORAMA Publishing House

Subject

Environmental Engineering

Reference17 articles.

1. 1. Barmintseva, A.E., Mugue, N.S. The Use of Microsatellite Loci for Identification of Sturgeon Species (Acipenseridae) and Hybrid Forms. Russian Journal of Genetics, 2013, vol. 49, no. 9, pp. 950-961. DOI: 10.7868/S0016675813090038.

2. 2. Barmintseva, A.E., Mugue, N.S. [Natural genetic polymorphism and phylogeography of siberian sturgeon Acipenser baerii brandt, 1869. Russian Journal of Genetics, 2017, vol. 53, no. 3, pp. 358-368 (in Russian). DOI: 10.7868/S001667581703002X.

3. 3. Vlasenko, S.A., Guteneva, G.I., Fomin, S.S. Ocenka jeffektivnosti estestvennogo vosproizvodstva osetrovyh na Nizhnej Volge [Assessment of condition of spawning grounds and efficiency of natural reproduction of sturgeons in the lower Volga]. Fishing issues, 2012, v. 13, no. 52, pp. 736-753 (in Russian).

4. 4. Guteneva, G.I., Fomin, S.S., Dedikova, T.N. The influence of the Volga flow on natural reproduction of sturgeon. Fisheries, 2015, no. 3, pp. 103-105 (in Russian).

5. 5. Kozlova, N.V., Makarova, E.G. Genetic research of young sterlet sturgeon (Acipenser ruthenus L., 1758.) and its hybrids in the lower spawning zones of the Volga river. Fisheries, 2018, no. 1, pp. 54-59 (in Russian).