Analysis of the expression level of circulating micrornas as biomarkers for early diagnosis of vascular calcification in coronary heart disease

Abstract

Purpose: Analysis of the expression level of circulating microRNAs in the blood as diagnostic biomarkers for the early diagnosis of vascular calcification in coronary heart disease. 36 patients took part in the study, including 18 patients suffering from coronary heart disease and calcification (average age: 45.7±6.5) and 18 healthy patients who did not have calcification as a control group (average age: 59.6±8.2). Bioinformatic analysis of data from blood samples (microRNA profiles) obtained from the public database GEO (GSE28858) and PubMed (PMC4964495) was carried out. Analysis of the level of microRNA expression was done using the microarray method. Statistical analysis was performed using R methods and using J-express Pro algorithms. We found previously shown relation of two microRNAs that were statistically significantly changed in our analysis with calcification of the cardiovascular system, mir-199a-5p and mir-382. We concluded that patients with coronary artery disease have several differentially expressed microRNA markers that can affect calcification in different ways. The data provided may have a positive result for the cardiovascular diagnostic strategy and personalized medicine.