Assessment of the effects of fungicide (Thiram) on somatic cells of broad bean (Vicia faba L.)

Abstract

Present study was carried out to know the impact of fungicide (Thiram) on somatic cells of broad bean (Vicia faba L.). Thiram is one of the most effective fungicide. Significant inhibition of mitotic index and increase in the frequencies of chromosome aberrations were observed. Results of the study indicate that both the plant bioassay found to be sensitive indicators for the genotoxicity assessment as the outcome of majority test system. Using plant bioassays for testing and monitoring environmental chemicals or pollution has many advantages. The mitotic index decreases due to exposure of plant extract with thiram in higher concentration as 150, 300, 550, 800 ppm for 8h and show reducing effect that is 21.00, 20.70, 18.61 and 16.77 in comparison to control that is 21.19. The higher concentrations of fungicide showed the genotoxic effects and damage the chromosomal integrity.