Antimycotic activity of green tea phytocompounds against Candida glabrata

Abstract

One of the medically important opportunistic fungal pathogen for humans is Candida glabrata that causes various types of candidiasis. Its environmental adaptations and antimicrobial resistance is now a great concern for public health. In the present study, the green tea phytocompounds; EGCg, Chlorogenic acid, Coumaroyl quinic acid and Rutin trihydrate along with a known antimycotic Fluconazole were studied for their antimycotic activity against Candida glabrata. The MIC90 for C. glabrata was observed at 125µg/ml for EGC g, 250 µg/mlf or Chlorogenic acid, 500µg/ml for Coumaroyl quinic acid and Rutin trihydrate while 12.5µg/ml for Fluconazole in macro dilution assay while the MFC values were 1000 µg/ml for EGC g, 500 µg/ml for Chlorogenic acid, Coumaroyl quinic acid, Rutin trihydrate and 50 µg/ml for Fluconazole. In microdilution assay, the MIC90 for C. glabrata was observed 125µg/ml for EGC g and chlorogenic acid, 500µg/ml for Coumaroyl quinic acid, Rutin trihydrate and 12.5µg/ml for Fluconazole while the MFC values were 31.25 µg/ml for Fluconazole, 250 µg/ml for chlorogenic acid and 500 µg/ml for EGC g, Coumaroyl quinic acid and Rutin trihydrate. EGCg and Chlorogenic acid was found to be more effective against C. glabrata and therefore these two were used for synergistic study along with Fluconazole. The viability of HeLa cells (in per cent) was observed ≥100% green tea phyto compounds. The viability of treated cells (in per cent) with a combination of Green tea, phytocompounds and fluconazole was observed between ≥98± 0.79 to ≥ 98± 0.87. Green tea phytocompounds mainly EGC g and chlorogenic acid can be used as synergistic molecules having antimycotic activity against C. glabrata.