Electrophoretic extraction of protein complexes after separation and detection by a combined method of non-denaturing two-dimensional electrophoresis and reversible staining-Reference-Cited by-同舟云学术

Electrophoretic extraction of protein complexes after separation and detection by a combined method of non-denaturing two-dimensional electrophoresis and reversible staining

Published:2022 Issue:1 Volume:66 Page:5-11
ISSN:1349-9394
Container-title:Journal of Electrophoresis
language:en
Short-container-title:J. Electrophor

Author:

Nakao Karin¹,Shimazaki Youji¹

Affiliation:

1. Faculty of Science, Ehime University

Publisher

Japanese Electrophoresis Society

Subject

General Chemical Engineering

Link

https://www.jstage.jst.go.jp/article/jelectroph/66/1/66_5/_pdf

Reference18 articles.

1. 1) Manabe T, Hayama E, Okuyama T. Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine. Clin Chem. 1982;28:824–827.

2. 2) Shimazaki Y, Sugawara Y, Ohtsuka Y, et al. Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis. Proteomics. 2003;3:2002–2007.

3. 3) Shimazaki Y, Manabe T. Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing electrophoresis after extraction with detergents. Biochim Biophys Acta. 2005;1749:95–101.

4. 4) Shimazaki Y, Shimizu K, Masaoka S. Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis. Talanta 2010;82:1063–1067.

5. 5) Chen H-M. Revisit of imidazole-zinc reverse stain for protein polyacrylamide gel electrophoresis. Methods Mol Biol. 2018;1853:139–147.

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