Development of diced electrophoresis gel assay for screening disease-related enzymatic activities
Author:
Affiliation:
1. Graduate School of Pharmaceutical Sciences, The University of Tokyo
Publisher
Japanese Electrophoresis Society
Link
https://www.jstage.jst.go.jp/article/electroph/65/2/65_29/_pdf
Reference10 articles.
1. 1) Komatsu T, Urano Y. Evaluation of enzymatic activities in living systems with smallmolecular fluorescent substrate probes. Anal Sci. 2015;31:257–265. doi:10.2116/analsci.31.257
2. 2) Komatsu T, Urano Y, Fujikawa Y, et al. Development of 2,6-carboxy-substituted boron dipyrromethene (BODIPY) as a novel scaffold of ratiometric fluorescent probes for live cell imaging. Chem Commun. 2009;7015–7017. doi:10.1039/b917209b
3. 3) Manchenko GP. Handbook Of Detection Of Enzymes On Electrophoretic Gels. CRC Press; 2002.
4. 4) Komatsu T, Hanaoka K, Adibekian A, et al. Diced electrophoresis gel assay for screening enzymes with specified activities. J Am Chem Soc. 2013;135:6002–6005. doi:10.1021/ja401792d
5. 5) Yoshioka K, Komatsu T, Nakada A, et al. Identification of tissue-restricted bioreaction suitable for in vivo targeting by fluorescent substrate library-based enzyme discovery. J Am Chem Soc. 2015;137:12187–12190. doi:10.1021/jacs.5b05884
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