STUDY OF STABILITY OF AVIAN INFECTIOUS BRONCHITIS VIRUS

Abstract

The sale, storage and use of vaccines sensitive to high and low temperatures is a problem that can be minimized by enhancing their stability and, as a consequence, improving the effectiveness of the preparation. Live lyophilized vaccines against avian infectious bronchitis are quite sensitive to environmental conditions. The stability of the preparation depends on a number of factors: the stability of the virus strain, the drying media (stabilizer), the mode of lyophilization and storage conditions of vaccines. One of the key components is the drying media, which provides protection during lyophilization, the stability of the preparation throughout the shelf life and its effectiveness during use. The aim of the study was to select the most promising drying media that ensure the stability of vaccines based on strains H-120 and VAR2/V2 during the required shelf life. The results of studies of the effect of different drying media (stabilizers) on the stability of live vaccines against avian infectious bronchitis from strains H-120 and VAR2/V2 at a temperature of 2-8°C and 37 °C. The results show that stabilizers, which include enzymatic peptone and polyvinylpyrrolidone (PVP) ensure the stability of live vaccines against IB from strains H-120 and VAR2/V2 for 18-24 months at a temperature of 2-8°C. The nature of lyophilisate formation, its quality and solubility were studied. The article publishes fragments of research related to the technology of production of live vaccines POLIMUN against avian infectious bronchitis (IB). In order to protect the technology, the authors presented the maximum possible data on the composition of drying media.