BLOOD PROTEIN SPECTRUM AFTER FEEDING RATS WITH METAL COMPLEXES IN THE COMPOSITION OF A POLYMER TRANSPORTER AND A NUTRIENT-DEFICIENT DIET

Abstract

The content of total protein and its spectrum in the blood were studied when rats were fed metal complexes (ME; Fe+2/+3-, Zn+2-, Cu+2 - and Mn+2-) as part of a polymer transporter (MEmLPEG400) and a diet deficient in nutrients. It was found that when fed a complete diet, the content of total protein in the blood plasma of rats is 107.5±3.0 g/l and is 15.6 - 17.8% higher in the experimental groups. At the same time, in the blood plasma of animals of the experimental groups, the content of γ-globulins is 2.3 - 2.9 times (р < 0.05 - 0.001) higher than in rats fed a complete diet, while the content of albumin, on the contrary, is reduced. The content of β-globulins is maximum (18.6±1.42%) in control animals and is 6.8 - 11.6% (p < 0.05 - 0.001) lower than when fed wheat groats and metals in mLPEG400. With the addition of Cu+2-mLPEG400 to the diet, the content of α-globulins in the blood plasma of rats increases by 10.8% (p < 0.01), and with Zn+2-mLPEG400 the content of prealbumin increases on 1.1 - 1.3% (p < 0.01), compared to the control and when feeding wheat groats. In the spectrum of lipoprotein fractions, the content of chylomicrons is lower by 10.3% (р < 0.05) after feeding wheat groats, however, with the addition of Mn+2-mLPEG400, on the contrary, the value is 13.6% (р < 0.05) higher, compared to the control. In the blood plasma of rats fed wheat groats and Cu+2- and Zn+2-mLPEG400, the content of very low-density lipoproteins, as well as low-density lipoproteins (p < 0.01 - 0.001), increased with the addition of Cu+2-mLPEG400, compared to animals for feeding a complete diet. In contrast, the content of high-density lipoproteins is 3.6% (р < 0.05) lower after feeding wheat groats and after adding metals in mLPEG400: Cu+2 – by 4.1% (р < 0.05), Fe +2/+3 – 4.2% (p < 0.05), Zn+2 – 3.2% (p < 0.05). The content of fatty acids adsorbed on albumin in the blood plasma of animals of the control group and when fed wheat groats does not differ (21.1 - 23.9%) and decreases by 7.1 – 12.7% (р < 0.05 - 0 .01) in rats that received metals in the composition of the polymer.