HISTOLOGICAL CHANGES IN EXPERIMENTAL UVEITIS IN RABBITS WITH STEM CELL INJECTIONS

Abstract

The article presents the results of histological examination of experimental uveitis in rabbits with the introduction of allogeneic mesenchymal stem cells. These research results make it possible to analyze and further study the effects of allogeneic mesenchymal stem cells in clinical cases of uveitis in animals. Uveitis is a symptom of many diseases that lead to slow functional and anatomical death of the eye. Uveitis causes inflammation of the middle (vascular) membrane of the eye, which consists of the choroid, ciliary body and iris. The use of complex therapy often smooths out the clinical picture of progressive intraocular inflammation, contributing to an increase in its latent period. All this with particular relevance points to the need to study the use of stem cells in eye diseases in animals. Our histological studies on the restoration of eye tissues from the introduced allogeneic mesenchymal stem cells indicate their effective use for uveitis in animals. Stem cells act as a regulator of proliferation in damaged eye tissues and cause cyto-differentiation during cell regeneration, activate the synthesis of anti-inflammatory mediators and enhance their own antioxidant properties. It was found that with the help of allogeneic mesenchymal stem cells, already on the 7th day of the experiment, a decrease in corneal stroma thickening was noted, and on the 14th day, restoration of the anterior surface epithelium was noted. Also on the 30th day of the experiment, almost complete restoration of damaged tissue structures of the eye and the end of the inflammatory process were noted. That is, histological studies indicate not only the recovery function of damaged tissue structures with the help of allogeneic mesenchymal stem cells, but also the effect on the intensity of the inflammatory process, which significantly reduces the time of repair of eye tissues at the level of cells and tissues. The obtained data using stem cells can be used for new modern methods of treating many eye pathologies in ophthalmology.