Disruption of day‐to‐night changes in circadian gene expression with chronic tendinopathy

Author:

Yeung Ching‐Yan Chloé12ORCID,Svensson René B.12,Yurchenko Kateryna12,Malmgaard‐Clausen Nikolaj M.12,Tryggedsson Ida12,Lendal Marius12ORCID,Jokipii‐Utzon Anja12,Olesen Jens L.12,Lu Yinhui3,Kadler Karl E.3,Schjerling Peter12ORCID,Kjær Michael12ORCID

Affiliation:

1. Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery Copenhagen University Hospital – Bispebjerg and Frederiksberg Copenhagen Denmark

2. Center for Healthy Aging, Department of Clinical Medicine University of Copenhagen Denmark

3. Wellcome Centre for Cell‐Matrix Research, Faculty of Biology, Medicine and Health University of Manchester Manchester UK

Abstract

AbstractOveruse injury in tendon tissue (tendinopathy) is a frequent and costly musculoskeletal disorder and represents a major clinical problem with unsolved pathogenesis. Studies in mice have demonstrated that circadian clock‐controlled genes are vital for protein homeostasis and important in the development of tendinopathy. We performed RNA sequencing, collagen content and ultrastructural analyses on human tendon biopsies obtained 12 h apart in healthy individuals to establish whether human tendon is a peripheral clock tissue and we performed RNA sequencing on patients with chronic tendinopathy to examine the expression of circadian clock genes in tendinopathic tissues. We found time‐dependent expression of 280 RNAs including 11 conserved circadian clock genes in healthy tendons and markedly fewer (23) differential RNAs with chronic tendinopathy. Further, the expression of COL1A1 and COL1A2 was reduced at night but was not circadian rhythmic in synchronised human tenocyte cultures. In conclusion, day‐to‐night changes in gene expression in healthy human patellar tendons indicate a conserved circadian clock as well as the existence of a night reduction in collagen I expression. imageKey points Tendinopathy is a major clinical problem with unsolved pathogenesis. Previous work in mice has shown that a robust circadian rhythm is required for collagen homeostasis in tendons. The use of circadian medicine in the diagnosis and treatment of tendinopathy has been stifled by the lack of studies on human tissue. Here, we establish that the expression of circadian clock genes in human tendons is time dependent, and now we have data to corroborate that circadian output is reduced in diseased tendon tissues. We consider our findings to be of significance in advancing the use of the tendon circadian clock as a therapeutic target or preclinical biomarker for tendinopathy.

Funder

Wellcome Trust

Publisher

Wiley

Subject

Physiology

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