Author:
Holichenko O.A., ,Shtemenko N.I.,Ovcharenko A.A.,Shtemenko A.V.
Abstract
We report about the interactions of dirhenium(III) compound cis-[Re2(Trp)2Cl4(CH3CN)2]Cl2 (I) with bovine serum protein (BSA) and guanine (G4) quadruplexes DNA by UV-Vis titration. Addition of I to BSA led to the interaction between these compounds with binding constant 5.6103 M–1 and hyperchromism (20.9%) of the main protein absorption band (280 nm). These results support our assumption about formation of the additional conjugated systems during the process of interaction with BSA. Stabilization of the quadruple bonded rhenium(III) complex compound was shown in the presence of BSA (the rate of destruction was reduced), that may be explained by interaction between amino acid residues of BSA and quadruple bond of dirhenium(III) complex compound. In addition, we have obtained data about strong hyperchromism (up to 100%) and significant shift of the maximum of absorption (blue shift) towards UV (2–9 nm) and visible (22 nm) regions in the spectra of mixtures G4s and I, that, in our opinion, correlated with a conformational change in DNA and with formation of additional conjugated systems around quadruple bond of I. In a whole, our work confirms the strong binding activity of a cluster dirhenium(III) compound towards G4 quadruplexes, that exceed the binding activity to proteins and witness to preferential interactions of I with G4 DNA in a living cell. These results may be used in DNA "silencing technology" and "antisense therapy".
Publisher
SHEI Ukrainian State University of Chemical Technology
Subject
Materials Chemistry,General Chemical Engineering,Environmental Chemistry,General Chemistry
Cited by
1 articles.
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