Metabolic Profiling and Antibacterial Assessment of Calotropis procera extract against Streptococcus group B

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Abstract

Calotropis procera is shrub of Asclepiadaceae family and is a source of antimicrobial metabolites against a greater range of bacteria including Streptococcus Group B (SGB). In the present study, different metabolic compounds were extracted from C. procera using 75% methanol. The extract of C. procera was assessed against different bacteria of the SGB family by estimating the zone of inhibition. Moreover, minimal inhibitory concentration (MIC) of different concentrations e.g., 0.001, 0.01, 0.1, 1.0 and 10 mg/mL were determined through zone of inhibition. Gas chromatography mass spectrometry (GC-MS) analysis of plant extract revealed 24 compounds. The most important compounds of the GC-MS array were including undecane, terephthalic acid, Cyclohexane, dimethyl-propane-thiosulfinate, Fluorobenzoic acid Octadecenoic acid and others. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound. The molecular characterization of different bacteria of SGB used in the study revealed the bacteria were consisted of antibiotic resistant genes against.

Publisher

Abasyn University

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