Development of New SSR Markers in Lentil by Scanning AG and AC Enriched Libraries with TG and TC Repeats-Reference-Cited by-同舟云学术

Development of New SSR Markers in Lentil by Scanning AG and AC Enriched Libraries with TG and TC Repeats

Published:2022-09-19 Issue: Volume: Page:
ISSN:2148-2683
Container-title:European Journal of Science and Technology
language:tr
Short-container-title:EJOSAT

Author:

DEMİR Şehriban¹,BAKIR Melike²

Affiliation:

1. ERCİYES ÜNİVERSİTESİ

2. ERCIYES UNIVERSITY

Abstract

Simple sequence repeats (SSRs) markers are important molecular tools widely used in genetic and genomic research in plants. However, the number of SSR markers developed in lentils (Lens culinaris Medik.) so far, which is an economically important cool season legume plant, has been very limited. The lack of SSR markers is among the main factors limiting lentil molecular breeding studies. In this study, 288 clones belonging to the enriched libraries with AC and AG repeats were screened by TG and TC repeats to develop SSR markers in lentil plants and 15 new SSR markers were developed. A total of 18 alleles were generated from markers showing polymorphism, and the Lc-MCu54 marker was identified as the most polymorphic marker. These developed markers are capable of contributing to many molecular-based studies in lentil plants.

Publisher

European Journal of Science and Technology

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference27 articles.

1. Andeden, E.E., Baloch, F.S., Çakır, E., Toklu, F., & Özkan, H. (2015). Development, characterization and mapping of microsatellite markers for lentil (Lens culinaris Medik.). Plant Breeding 134, 589–598.

2. Arumuganathan, K., & Earle, E.D., (1991). Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9, 208–218.

3. Bahl, P.N., Lal, S., & Sharma, B.M. (1993). Page: 1-10. “An overview of the production and problems in southeast Asia”. In: Lentil in South Asia. Proceedings of the seminar on lentils in South Asia, ICARDA. Editors: Erskine, W., Saxena, M.C. Aleppo, Syria.

4. Bakır, M., & Kahraman, A. (2019) Development of new SSR (simple sequence repeat) markers for lentils (Lens culinaris Medik.) from genomic library enriched with AG and AC microsatellites. Biochemical Genetics 57, 338-353.

5. Bloor, P.A., Barker, F.S., Watts, P.C., Noyes, H.A., & Kemp, S.J, (2001). Microsatellite libraries by enrichment. https://www.genomics.liv.ac.uk/animal/RESEARCH/MICROSAT.PDF Accessed 10 April 2018.

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. INTEGRATION OF NOVEL SSR MARKERS INTO THE LENTIL (Lens culinaris Medik.) GENOME;Turkish Journal Of Field Crops;2024-06-21