Methylated DNA Collected by Tampons—A New Tool to Detect Endometrial Cancer-Reference-Cited by-同舟云学术

Methylated DNA Collected by Tampons—A New Tool to Detect Endometrial Cancer

Published:2004-05-01 Issue:5 Volume:13 Page:882-888
ISSN:1055-9965
Container-title:Cancer Epidemiology, Biomarkers & Prevention
language:en
Short-container-title:

Author:

Fiegl Heidi¹,Gattringer Conny¹,Widschwendter Andreas¹,Schneitter Alois¹,Ramoni Angela¹,Sarlay Daniela¹,Gaugg Inge¹,Goebel Georg²,Müller Hannes M.¹,Mueller-Holzner Elisabeth¹,Marth Christian¹,Widschwendter Martin¹

Affiliation:

1. 1Department of Obstetrics and Gynecology, Innsbruck University Hospital and

2. 2Department of Biostatistics and Documentation, University of Innsbruck, Innsbruck, Austria

Abstract

Abstract This proof of principle study aimed to define a new and simple strategy for detection of endometrial cancer using epigenetic markers. We investigated DNA isolated from vaginal secretion collected from tampon for aberrant methylation of five genes (CDH13, HSPA2, MLH1, RASSF1A, and SOCS2) using MethyLight in 15 patients with endometrial cancer and 109 patients without endometrial cancer. All endometrial cancer patients revealed three or more methylated genes, whereas 91% (99 of 109) of the patients without endometrial cancer had no or fewer than three genes methylated in their vaginal secretion. The methods developed in this study provide the basis for a prospective clinical trial to screen asymptomatic women who are at high risk for endometrial cancer.

Publisher

American Association for Cancer Research (AACR)

Subject

Oncology,Epidemiology

Link

https://aacrjournals.org/cebp/article-pdf/13/5/882/2262541/882-888.pdf

Reference18 articles.

1. Parkin DM, Pisani P, Ferlay J. Estimates of the worldwide incidence of 25 major cancers in 1990. Int J Cancer 1999;80:827-41.

2. Vasen HF, Wijnen JT, Menko FH, et al. Cancer risk in families with hereditary nonpolyposis colorectal cancer diagnosed by mutation analysis. Gastroenterology 1996;110:1020-7.

3. Smith RA, von Eschenbach AC, Wender R, et al. American Cancer Society guidelines for the early detection of cancer: update of early detection guidelines for prostate, colorectal, and endometrial cancers. Also: Update 2001—testing for early lung cancer detection. CA Cancer J Clin 2001;51:38-75.

4. Langer RD, Pierce JJ, O'Hanlan KA, et al. Transvaginal ultrasonography compared with endometrial biopsy for the detection of endometrial disease. Postmenopausal Estrogen/Progestin Interventions Trial. N Engl J Med 1997;337:1792-8.

5. Al Jehani RM, Jeyarajah AR, Hagen B, et al. Model for the molecular genetic diagnosis of endometrial cancer using K-ras mutation analysis. J Natl Cancer Inst 1998;90:540-2.

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Strategies of Early Diagnosis of Cancers;Handbook of Oncobiology: From Basic to Clinical Sciences;2024

2. Performance of the WID-qEC test versus sonography to detect uterine cancers in women with abnormal uterine bleeding (EPI-SURE): a prospective, consecutive observational cohort study in the UK;The Lancet Oncology;2023-12

3. Methylated DNA Markers for Sporadic Colorectal and Endometrial Cancer Are Strongly Associated with Lynch Syndrome Cancers;Cancer Prevention Research;2023-09-20

4. Evaluation of somatic mutations in cervicovaginal samples as a non-invasive method for the detection and molecular classification of endometrial cancer;eBioMedicine;2023-08

5. MiR-532-3pinhibited the methylation ofSOCS2to suppress the progression of PC by targetingDNMT3A;Life Science Alliance;2023-04-21