Downregulation of BASP1 Promotes Temozolomide Resistance in Gliomas via Epigenetic Activation of the FBXO32/NF-κB/MGMT Axis

Author:

Liao Xinyi12ORCID,Li Ziwen12ORCID,Zheng Haiqing1ORCID,Qian Wanying12ORCID,Zhang Shuxia3ORCID,Chen Suwen12ORCID,Li Xincheng12ORCID,Tang Miaoling3ORCID,Xu Yingru3ORCID,Yu Ruyuan3ORCID,Li Man12ORCID,Song Libing4ORCID,Li Jun12ORCID

Affiliation:

1. 1Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

2. 2Department of Biochemistry, Zhongshan school of medicine, Sun Yat-sen University, Guangzhou, China.

3. 3Department of Oncology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

4. 4State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Abstract

Abstract The chemoresistance of temozolomide-based therapy is a serious limitation for lasting effective treatment of gliomas, while the underlying mechanisms remain unclear. In this study, we showed that downregulation of BASP1 correlated negatively with the response to temozolomide therapy and disease-free survival (DFS) of patients with gliomas. Silencing BASP1 significantly enhanced the temozolomide resistance of glioma cells both in vitro and in vivo through repair of temozolomide-induced DNA damage via activation of the FBXO32/NF-κB/MGMT axis in both MGMT-methylated and -unmethylated gliomas. We demonstrated that loss of BASP1 resulted in removal of TRIM37/EZH2 complex–induced repressive histone modifications, including H2A-ub and H3K27me3, but addition of WDR5/MLL complex–mediated active histone modifications, including H3K4me3 and H3K9ac, on the FBXO32 promoter, which elicited in FBXO32 upregulation and further activated NF-κB/MGMT signaling via ubiquitin-dependent degradation of IκBα. Importantly, treatment with OICR-9429, an antagonist of the WDR5–MLL interaction, impaired the FBXO32/NF-κB/MGMT axis–mediated repair of temozolomide-induced DNA damage, leading to significant apoptosis of BASP1-downregulated glioma cells. These findings shed light on the molecular mechanism underlying BASP1-mediated epigenetic transcriptional repression and may represent a potential strategy in the fight against temozolomide-resistant gliomas. Implications: BASP1 downregulation promotes temozolomide resistance in gliomas through WDR5/MLL complex–mediated epigenetic activation of the FBXO32/NF-κB/MGMT axis, providing new target for improving outcomes in patients with temozolomide-resistant gliomas.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

American Association for Cancer Research (AACR)

Subject

Cancer Research,Oncology,Molecular Biology

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