REN C-5312T, but not C-5434T, correlates to increase serum angiotensin I level in angiotensin II receptor blockers-treated hypertensive patients
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Published:2016-05-16
Issue:2
Volume:11
Page:552
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ISSN:1991-0088
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Container-title:Bangladesh Journal of Pharmacology
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language:
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Short-container-title:Bangladesh J Pharmacol
Author:
Rohman Mohammad Saifur,
Fahrany Frastiqa,
Maslahah Imama,
Widodo -,
Lukitasari Mifetika,
Kurniawan AdityaORCID,
Endharti Agustina Tri
Abstract
<p class="Abstract">Renin distal enhancer plays a crucial role in regulating renin gene (REN) expression. REN C-5312T enhancer<span lang="IN"> polymorphism increased enhancer activity. REN C-5434T was also identified which supposed to part of enhancer region. Therefore, this study aimed to investigate contribution of both C-5312T and C-5434T to serum angiotensin I in response to angiotensin II receptor blockers (ARB). C-5312T was identified in 46 hypertensive patients by using multiplex polymerase chain reaction (PCR), while C-5434T by using PCR-restiction fragment length of polymorphism (RFLP). Angiotensin I was measured using enzyme linked immunosorbent assay (ELISA). A significant difference of baseline angiotensin I was observed between -5312CC and -5312CT/TT (p=0.038) as well as the angiotensin I after 5 months of ARB treatment (p=0.008) in -5434CT/TT population, but not REN C-5434T. In conclusion, C-5312T resulted in increased mRNA renin level as consequence of higher enhancer activity not only at the baseline but also after 5 months ARB treatment.</span></p>
Publisher
Bangladesh Journals Online (JOL)