Antithrombotic potential of Berberis calliobotrys extract

Abstract

<p class="Abstract">This study was focused with the aim to investigate the antithrombotic potential of <em>Berberis </em>calliobotrys. Aqueous-methanolic extract and various fractions showed significant (p&lt;0.05-0.001) increase in prothrombin, activated partial thromboplastin and clotting time while only aqueous methanolic extract caused clot lysis when added to the blood samples of rabbit and human. <em>In vivo</em> study in rabbits, butanolic fraction (100 mg/kg) produced more significant prolongation in bleeding, prothrombin, activated partial thromboplastin and clotting time. Interestingly butanolic fraction had shown more pronounced effects among all tested extracts both<em> in vivo</em> and in vitro studies. Hence, it was subjected to antilipid peroxidation and phytochemical studies (total falvonoid contents, HPLC-DAD profile, FTIR). In conclusion, <em>B. </em>calliobotrys induce transient changes in the coagulation parameters, may it possess active constituents responsible for its antithrombotic potential.</p><p> </p>