In vitro neutralization of Eachis carinatus and Naja naja venom by Canthium parviflorum and its GC-MS analysis

Abstract

<p class="Abstract"><em>Canthium </em>parviflorum is used in the traditional therapy to treat snakebite victims. In the present study, partially purified methanol root extract of <em>C. </em>parviflorum was used for the neutralization of Eachis carinatus and <em>Naja naja</em> venom. The extract inhibited <em>in vitro</em> phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase, protease, phospholipase A₂and 5’nucleotidase activities of both venoms. One of the promising band exhibited neutralization of all the<em> in vitro</em> enzyme activities and was further subjected to GC-MS analysis which revealed the presence of eight active phytocompounds. These phytochemicals might be responsible for <em>in vitro</em> enzyme neutralization. Methanol extract possesses potent active phytochemicals against the site specific toxins and hydrolytic enzymes analyzed.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/oSLdFcfaJxU">Phospholipase A2 assay</a>: 1 min 36 sec</p>