Development of an Assay Method for Simultaneous Determination of Ciprofloxacin and Naproxen by UV Spectrophotometric Method

Abstract

The PDF for this article was updated on 18/11/2011.YM Jhanker and KB Sutradhar were added as authors to this paper on 18/11/2011.The Objective of the present study was to develop a simple, reproducible and economical spectrophotometric method for simultaneous determination of Ciprofloxacin and Naproxen. Both Drugs obey Beer's law in the range of 1-5μg/ml concentration. The Standard curve of Ciprofloxacin and Naproxen in the media of 0.1N HCl, Distilled water and Phosphate buffer are obtained by plotting absorbance versus concentration where calibration curve was found to be linear (R2>0.99) with optimum value of standard error for the entire analytical medium used. The plot of the residuals was normally distributed around the regression line, which reflects the accuracy of the method. For simultaneous determination of Ciprofloxacin and Naproxen, the linear plot was found in the media of distilled water and 0.1N HCl acid. The maximum absorbance was found in the media of distilled water for Ciprofloxacin at wavelength 278nm and for Naproxen at 228nm. The calibration curve was to be linear for Ciprofloxacin as R2 >0.99 and for Naproxen as R2 >0.99.The maximum absorbance was found in the media of 0.1N HCl for Ciprofloxacin at wavelength 277nm and for Naproxen at 228nm. The calibration curve was to be linear for Ciprofloxacin as R2 >0.98 and for Naproxen as R2 >0.99. Key words: Ciprofloxacin; Naproxen; Spectrophotometric analysis; Simultaneous equation method. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8876 SJPS 2011; 4(1): 84-90