Antimicrobial Sensitivity Pattern of Bacteria Isolated from Pus Sample Collected from a Private Diagnostic Laboratory in Rangpur District of Bangladesh

Abstract

Background: The pattern of pathogens isolated from pus culture and their antibiotic sensitivity reports are very crucial for the management of the patients. Objective: The objective of the study was to determine the bacteriological profiles and antimicrobial sensitivity pattern of bacteria isolated from pus cultures. Methodology: This retrospective study was conducted in a Private Diagnostic Lab, Rangpur, Bangladesh. The samples were collected from January 2020 to December 2020 for a period of one year. Pus samples were collected from patients in a sterile method and was sent to laboratory section for further procedure. The pus was inoculated on Blood agar media and MacConkey’s agar media for culture and identification was confirmed by biochemical tests. Antimicrobial sensitivity was done by Disc diffusion method on Mueller Hinton Agar media. Result: A total of 180 pus culture were analysed. Among 180 samples, culture positive was 140(77.8%). The most common age group was 18 to 40 years which was 80(44.4%) cases. Among the isolated bacteria Staphylococcus aureus was 34(24.3%), Escherichia coli 34(24.3%), Proteus species 32(22.9%), Pseudomonas aeruginosa 18(12.9%), Klebsiella pneumonia 10(7.1%), Streptococcus species 6(4.3%). Conclusion: In conclusion, the most common isolated bacteria are Staphylococcus aureus followed by Escherichia coli, Proteus species, Pseudomonas aeruginosa, Klebsiella pneumonia, with the resistant to majority of the commonly used antibiotics. Bangladesh Journal of Infectious Diseases December 2021;8(2):64-70