Anti-snake venom potential of Clerodendrum serratum extracts on Bungarus caeruleus and Daboia russelii venom

Abstract

<p class="Abstract">Anti-snake venom therapy is the only treatment for snake bite but leads to acute and chronic conditions which may be severe. The medicinal plants have gained importance over years to find an effective alternative to anti-snake venom. The present study focused on evaluating the potential of <em>Clerodendrum serratum</em> for the anti-snake venom activity. Phytochemicals were extracted from the <em>C. serratum</em> with different solvents. The ethyl acetate and methanolic extracts were found to neutralize the major enzyme toxins (phospholipase A₂, protease and hyaluronidase) of <em>Bungarus caeruleus</em> and <em>Daboia russelii</em> venom at a concentration of 100 µg/mL. The fibrinogenolytic activity of both the venoms were neutralized. The study proves that the plant <em>C. serratum</em> possesses certain compounds which inhibit the toxins present in the venom of <em>B. caeruleus</em> and <em>D. russelii</em>.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Hyaluronidase assay:   3 min  30 sec   <a href="https://www.youtube.com/v/Xz5C6bbwgW0">Full Screen</a>    <a href="https://www.youtube.com/watch?v=Xz5C6bbwgW0">Alternate</a></p>