α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots

Abstract

<p>Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC₅₀ values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC₅₀ = 4.9 mg/mL). Grandivitinol (IC₅₀ = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.</p><p><strong>Video Clip of Methodology</strong>:</p><p>8 min 26 sec:   <a href="https://www.youtube.com/v/x0gakPjgXmc">Full Screen</a>   <a href="https://www.youtube.com/watch?v=x0gakPjgXmc">Alternate</a></p><p> </p>