Development of a Quantification and Detection Method for 2-MIB-producing Cyanobacteria-Reference-Cited by-同舟云学术

Development of a Quantification and Detection Method for 2-MIB-producing Cyanobacteria

Published:2022-09-27 Issue:4 Volume:23 Page:
ISSN:1303-2712
Container-title:Turkish Journal of Fisheries and Aquatic Sciences
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Author:

Zhang Ji¹,Shen Qingyue¹,Miao Hanchen¹,Li Qintong²^ORCID,Shimada Marie³,Yuan Tian⁴,Utsumi Motoo⁴,Lei Zhongfang⁴^ORCID,Zhang Zhenya⁴,Takanashi Hirokazu⁵^ORCID,Fujimoto Naoshi⁶,Ichise Satoshi⁷,Asada Yasuhiro⁸,Nishimura Osamu⁹^ORCID,Akiba Michihiro⁸,Shimizu Kazuya²^ORCID

Affiliation:

1. University of Tsukuba, Graduate School of Science and Technology, 1-1-1 Tennoudai, Tsukuba Ibaraki Japan.

2. Tokyo University, Faculty of Life sciences, 1-1-1 Izumino Ora-gun Itakura Gunma Japan.

3. Water Quality Management Center, Ibaraki Prefectural Public Enterprise Bureau, 2972 Ooiwata, Tsuchiura, Ibaraki, Japan.

4. University of Tsukuba, Faculty of Life and Environmental Sciences, 1-1-1 Tennoudai, Tsukuba Ibaraki Japan.

5. Kagoshima University, Biotechnology and Chemical Engineering, Department of Chemistry, 1-21-40 Korimoto, Kagoshima City, Kagoshima, Japan.

6. Tokyo University of Agriculture, Faculty of Applied Bioscience, 1-1-1 Sakuragaoka, Setagaya-ku Tokyo, Japan.

7. Lake Biwa Environmental Research Institute, 5-34 Yanagigasaki, Otsu, Japan.

8. National Institute of Public Health, 2-3-6 Minami, Wako, Saitama, Japan.

9. Tohoku University, Department of Civil and Environmental Engineering, 6-6-06 Aramaki-Aza Aoba, Sendai, Miyagi, Japan.

Abstract

Consumers often complain about taste and odor (T&O) in drinking water and freshwater fishery. One of the common T&O compounds, 2-methylisoborneol (2-MIB), can be detected by humans even when the concentration is below 10 ng/L. A forecast method of T&O occurrence is required to control drinking water plants and fishery farms to determine the timing of exchange of activated carbon or exchange the water in earthy pond. Traditional monitoring methods such as PCR, microscopy, and chemical analysis require a long time, are high in cost, and have a complex operation. We conducted this study to develop whole-cell PCR and whole-cell qPCR assays for rapid detection and quantification of 2-MIB-producing cyanobacteria without DNA extraction to detect 2-MIB cyclase gene (mtc). Pseudanabaena foetida strain 1705-12 (Lake Kasumigaura), strain 1803-12 (Lake Kasumigaura), and strain PTG (Lake Biwa) of 2-MIB-producing cyanobacteria were used in the study. The positive correlation between the results of whole-cell PCR, whole-cell qPCR and chlorophyll a (Chl.a), and gene abundances illustrated that whole-cell PCR and whole-cell qPCR assays could rapidly and conveniently detect and quantify 2-MIB-producing cyanobacteria. Thus, this study provides a valuable tool for prediction of T&O events in drinking water and freshwater fishery.

Publisher

Central Fisheries Research Institute (SUMAE)

Subject

Animal Science and Zoology,Aquatic Science