Experimental Analysis of the Arabidopsis Mitochondrial Proteome Highlights Signaling and Regulatory Components, Provides Assessment of Targeting Prediction Programs, and Indicates Plant-Specific Mitochondrial Proteins
[W]
Author:
Heazlewood Joshua L.1, Tonti-Filippini Julian S.1, Gout Alexander M.1, Day David A.1, Whelan James1, Millar A. Harvey1
Affiliation:
1. Plant Molecular Biology Group, School of Biomedical and Chemical Sciences, University of Western Australia, Crawley 6009, Western Australia, Australia
Abstract
Abstract
A novel insight into Arabidopsis mitochondrial function was revealed from a large experimental proteome derived by liquid chromatography–tandem mass spectrometry. Within the experimental set of 416 identified proteins, a significant number of low-abundance proteins involved in DNA synthesis, transcriptional regulation, protein complex assembly, and cellular signaling were discovered. Nearly 20% of the experimentally identified proteins are of unknown function, suggesting a wealth of undiscovered mitochondrial functions in plants. Only approximately half of the experimental set is predicted to be mitochondrial by targeting prediction programs, allowing an assessment of the benefits and limitations of these programs in determining plant mitochondrial proteomes. Maps of putative orthology networks between yeast, human, and Arabidopsis mitochondrial proteomes and the Rickettsia prowazekii proteome provide detailed insights into the divergence of the plant mitochondrial proteome from those of other eukaryotes. These show a clear set of putative cross-species orthologs in the core metabolic functions of mitochondria, whereas considerable diversity exists in many signaling and regulatory functions.
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Reference79 articles.
1. Adams, K.L., Daley, D.O., Qiu, Y.L., Whelan, J., and Palmer, J.D. (2000). Repeated, recent and diverse transfers of a mitochondrial gene to the nucleus in flowering plants. Nature 408
, 354–357. 2. Adams, K.L., Daley, D.O., Whelan, J., and Palmer, J.D. (2002). Genes for two mitochondrial ribosomal proteins in flowering plants are derived from their chloroplast or cytosolic counterparts. Plant Cell 14
, 931–943. 3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990). Basic local alignment search tools. J. Mol. Biol. 215
, 403–410. 4. Andersson, S.G., Zomorodipour, A., Andersson, J.O., Sicheritz-Ponten, T., Alsmark, U.C., Podowski, R.M., Naslund, A.K., Eriksson, A.S., Winkler, H.H., and Kurland, C.G. (1998). The genome sequence of Rickettsia prowazekii and the origin of mitochondria. Nature 396
, 133–140. 5. Babiychuk, E., Fuangthong, M., Van Montagu, M., Inze, D., and Kushnir, S. (1997). Efficient gene tagging in Arabidopsis thaliana using a gene trap approach. Proc. Natl. Acad. Sci. USA 94
, 12722–12727.
Cited by
503 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|