Affiliation:
1. Heinrich-Heine-Universität Düsseldorf, Institut für Entwicklungs- und Molekularbiologie der Pflanzen, 40225 Duesseldorf, Germany
2. Universität Rostock, Abteilung Pflanzenphysiologie, 18059 Rostock, Germany
Abstract
Abstract
The mitochondrial Gly decarboxylase complex (GDC) is a key component of the photorespiratory pathway that occurs in all photosynthetically active tissues of C3 plants but is restricted to bundle sheath cells in C4 species. GDC is also required for general cellular C1 metabolism. In the Asteracean C4 species Flaveria trinervia, a single functional GLDP gene, GLDPA, encodes the P-subunit of GDC, a decarboxylating Gly dehydrogenase. GLDPA promoter reporter gene fusion studies revealed that this promoter is active in bundle sheath cells and the vasculature of transgenic Flaveria bidentis (C4) and the Brassicacean C3 species Arabidopsis thaliana, suggesting the existence of an evolutionarily conserved gene regulatory system in the bundle sheath. Here, we demonstrate that GLDPA gene regulation is achieved by an intricate interplay of transcriptional and posttranscriptional mechanisms. The GLDPA promoter is composed of two tandem promoters, PR2 and PR7, that together ensure a strong bundle sheath expression. While the proximal promoter (PR7) is active in the bundle sheath and vasculature, the distal promoter (PR2) drives uniform expression in all leaf chlorenchyma cells and the vasculature. An intron in the 5′ untranslated leader of PR2-derived transcripts is inefficiently spliced and apparently suppresses the output of PR2 by eliciting RNA decay.
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Cited by
41 articles.
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