Phosphoenolpyruvate Provision to Plastids Is Essential for Gametophyte and Sporophyte Development inArabidopsis thaliana-Reference-Cited by-同舟云学术

Phosphoenolpyruvate Provision to Plastids Is Essential for Gametophyte and Sporophyte Development inArabidopsis thaliana

Published:2010-08-01 Issue:8 Volume:22 Page:2594-2617
ISSN:1532-298X
Container-title:The Plant Cell
language:en
Short-container-title:

Author:

Prabhakar Veena¹,Löttgert Tanja¹,Geimer Stefan²,Dörmann Peter³,Krüger Stephan¹,Vijayakumar Vinod¹,Schreiber Lukas⁴,Göbel Cornelia⁵,Feussner Kirstin⁶,Feussner Ivo⁵,Marin Kay⁷,Staehr Pia¹,Bell Kirsten¹,Flügge Ulf-Ingo¹,Häusler Rainer E.¹

Affiliation:

1. Universität zu Köln, Biozentrum, Botanisches Institut II, D-50674 Cologne, Germany

2. Universität Bayreuth, Zellbiologie/Elektronenmikroskopie NW I/B1, D-95447 Bayreuth, Germany

3. Universität Bonn, Molekulare Biotechnologie, D-53115 Bonn, Germany

4. Universität Bonn, Institut für Zelluläre und Molekulare Botanik, Ecophysiology of Plants, D-53115 Bonn, Germany

5. Georg August University, Albrecht von Haller Institute for Plant Sciences, Ernst Caspari Building, Department of Plant Biochemistry, D-37077 Goettingen, Germany

6. Georg August University, Institute for Microbiology and Genetics, Department of Molecular Microbiology and Genetics, D-37077 Goettingen, Germany

7. Universität zu Köln, Institut für Biochemie, D-50674 Cologne, Germany

Abstract

AbstractRestriction of phosphoenolpyruvate (PEP) supply to plastids causes lethality of female and male gametophytes in Arabidopsis thaliana defective in both a phosphoenolpyruvate/phosphate translocator (PPT) of the inner envelope membrane and the plastid-localized enolase (ENO1) involved in glycolytic PEP provision. Homozygous double mutants of cue1 (defective in PPT1) and eno1 could not be obtained, and homozygous cue1 heterozygous eno1 mutants [cue1/eno1(+/−)] exhibited retarded vegetative growth, disturbed flower development, and up to 80% seed abortion. The phenotypes of diminished oil in seeds, reduced flavonoids and aromatic amino acids in flowers, compromised lignin biosynthesis in stems, and aberrant exine formation in pollen indicate that cue1/eno1(+/−) disrupts multiple pathways. While diminished fatty acid biosynthesis from PEP via plastidial pyruvate kinase appears to affect seed abortion, a restriction in the shikimate pathway affects formation of sporopollonin in the tapetum and lignin in the stem. Vegetative parts of cue1/eno1(+/−) contained increased free amino acids and jasmonic acid but had normal wax biosynthesis. ENO1 overexpression in cue1 rescued the leaf and root phenotypes, restored photosynthetic capacity, and improved seed yield and oil contents. In chloroplasts, ENO1 might be the only enzyme missing for a complete plastidic glycolysis.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Plant Science

Link

http://academic.oup.com/plcell/article-pdf/22/8/2594/36931362/plcell_v22_8_2594.pdf

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