Phytochrome Phosphorylation Modulates Light Signaling by Influencing the Protein–Protein Interaction[W]-Reference-Cited by-同舟云学术

Phytochrome Phosphorylation Modulates Light Signaling by Influencing the Protein–Protein Interaction[W]

Published:2004-10-01 Issue:10 Volume:16 Page:2629-2640
ISSN:1532-298X
Container-title:The Plant Cell
language:en
Short-container-title:

Author:

Kim Jeong-Il¹²³,Shen Yu²,Han Yun-Jeong²,Park Joung-Eun¹³,Kirchenbauer Daniel⁴,Soh Moon-Soo¹,Nagy Ferenc⁵,Schäfer Eberhard⁴,Song Pill-Soon¹²³

Affiliation:

1. Kumho Life and Environmental Science Laboratory, Gwangju 500-712, Korea

2. Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588-0304

3. Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea

4. Institut für Biologie II/Botanik, Universität Freiburg, D-79104 Freiburg, Germany

5. Institute of Plant Biology, Biological Research Center, H6701 Szeged, Hungary

Abstract

Abstract Plant photoreceptor phytochromes are phosphoproteins, but the question as to the functional role of phytochrome phosphorylation has remained to be elucidated. We investigated the functional role of phytochrome phosphorylation in plant light signaling using a Pfr-specific phosphorylation site mutant, Ser598Ala of oat (Avena sativa) phytochrome A (phyA). The transgenic Arabidopsis thaliana (phyA-201 background) plants with this mutant phyA showed hypersensitivity to light, suggesting that phytochrome phosphorylation at Serine-598 (Ser598) in the hinge region is involved in an inhibitory mechanism. The phosphorylation at Ser598 prevented its interaction with putative signal transducers, Nucleoside Diphosphate Kinase-2 and Phytochrome-Interacting Factor-3. These results suggest that phosphorylation in the hinge region of phytochromes serves as a signal-modulating site through the protein–protein interaction between phytochrome and its putative signal transducer proteins.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Plant Science

Link

http://academic.oup.com/plcell/article-pdf/16/10/2629/36875569/plcell_v16_10_2629.pdf

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