Silencing of Hydroxycinnamoyl-Coenzyme A Shikimate/Quinate Hydroxycinnamoyltransferase Affects Phenylpropanoid Biosynthesis[W]

Author:

Hoffmann Laurent1,Besseau Seébastien1,Geoffroy Pierrette1,Ritzenthaler Christophe1,Meyer Denise1,Lapierre Catherine2,Pollet Brigitte2,Legrand Michel1

Affiliation:

1. Institut de Biologie Moleéculaire des Plantes, Uniteé Propre de Recherche, 2357 du Centre National de la Recherche Scientifique, Universiteé Louis Pasteur, 67000 Strasbourg, France

2. Laboratoire de Chimie Biologique, Uniteé Mixte de Recherche 206, Institut National de la Recherche Agronomique-Institut National Agronomique, 78850 Thiverval-Grignon, France

Abstract

Abstract The hydroxyl group in the 3-position of the phenylpropanoid compounds is introduced at the level of coumarate shikimate/quinate esters, whose synthesis implicates an acyltransferase activity. Specific antibodies raised against the recombinant tobacco (Nicotiana tabacum) acyltransferase revealed the accumulation of the enzyme in stem vascular tissues of tobacco, in accordance with a putative role in lignification. For functional analysis, the acyltransferase gene was silenced in Arabidopsis thaliana and N. benthamiana by RNA-mediated posttranscriptional gene silencing. In Arabidopsis, gene silencing resulted in a dwarf phenotype and changes in lignin composition as indicated by histochemical staining. An in-depth study of silenced N. benthamiana plants by immunological, histochemical, and chemical methods revealed the impact of acyltransferase silencing on soluble phenylpropanoids and lignin content and composition. In particular, a decrease in syringyl units and an increase in p-hydroxyphenyl units were recorded. Enzyme immunolocalization by confocal microscopy showed a correlation between enzyme accumulation levels and lignin composition in vascular cells. These results demonstrate the function of the acyltransferase in phenylpropanoid biosynthesis.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Plant Science

Reference52 articles.

1. Abbott, J.C., Barakate, A., Pinçon, G., Legrand, M., Lapierre, C., Mila, I., Schuch, W., and Halpin, C. (2002). Simultaneous suppression of multiple genes by single transgenes. Down-regulation of three unrelated lignin biosynthetic genes in tobacco. Plant Physiol.  128  ,  844–853.

2. Atanassova, R., Favet, N., Martz, F., Chabbert, B., Thollier, M.T., Monties, B., Fritig, B., and Legrand, M. (1995). Altered lignin composition in transgenic tobacco expressing O-methyltransferase sequences in sense and antisense orientation. Plant J.  8  ,  465–477.

3. Bandoniene, D., and Murkovic, M. (2002). On-line HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.). J. Agric. Food Chem.  50  ,  2482–2487.

4. Bechtold, N., Ellis, J., and Pelletier, G. (1993). In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. III Sci. Vie  316  ,  1194–1199.

5. Binns, A.N., Chen, R.H., Wood, H.N., and Lynn, D.G. (1987). Cell division promoting activity of naturally occurring dehydrodiconiferyl glucosides: Do cell wall components control cell division? Proc. Natl. Acad. Sci. USA  84  ,  980–984.

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