The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa
[W]
Author:
Yamaguchi Takahiro1, Nagasawa Nobuhiro1, Kawasaki Shinji2, Matsuoka Makoto3, Nagato Yasuo1, Hirano Hiro-Yuki1
Affiliation:
1. Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan 2. National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-0856, Japan 3. Bioscience and Biotechnology Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
Abstract
Abstract
In this article, we report that carpel specification in the Oryza sativa (rice) flower is regulated by the floral homeotic gene DROOPING LEAF (DL) that is distinct from the well-known ABC genes. Severe loss-of-function mutations of DL cause complete homeotic transformation of carpels into stamens. Molecular cloning reveals that DL is a member of the YABBY gene family and is closely related to the CRABS CLAW (CRC) gene of Arabidopsis thaliana. DL is expressed in the presumptive region (carpel anlagen), where carpel primordia would initiate, and in carpel primordia. These results suggest that carpel specification is regulated by DL in rice flower development. Whereas CRC plays only a partial role in carpel identity, DL may have been recruited to have the more essential function of specifying carpels during the evolution of rice. We also show that DL interacts antagonistically with class B genes and controls floral meristem determinacy. In addition, severe and weak dl alleles fail to form a midrib in the leaf. The phenotypic analysis of dl mutants, together with analyses of the spatial expression patterns and ectopic expression of DL, demonstrate that DL regulates midrib formation by promoting cell proliferation in the central region of the rice leaf.
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Reference35 articles.
1. Alvarez, J., and Smyth, D.R. (1999). CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel development in parallel with AGAMOUS. Development 126
, 2377–2386. 2. Ambrose, B.A., Lerner, D.R., Ciceri, P., Padilla, C.M., Yanofsky, M.F., and Schmidt, R.J. (2000). Molecular and genetic analyses of the silky1 gene reveal conservation in floral organ specification between eudicots and monocots. Mol. Cell 5
, 569–579. 3. Bowman, J.L. (2000). The YABBY gene family and abaxial cell fate. Curr. Opin. Plant Biol. 3
, 17–22. 4. Bowman, J.L., Baum, S.F., Eshed, Y., Putterill, J., and Alvarez, J. (1999). Molecular genetics of gynoecium development in Arabidopsis. Curr. Top. Dev. Biol. 45
, 155–205. 5. Bowman, J.L., Sakai, H., Jack, T., Weigel, D., Mayer, U., and Meyerowitz, E.M. (1992). SUPERMAN, a regulator of floral homeotic genes in Arabidopsis. Development 114
, 599–615.
Cited by
374 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|