Alpha Lipoic Acid Ameliorates the Artificial Sugar Induced Injury to the Female Reproductive Organs of Mice

Abstract

Background: High Fructose Corn syrup (HFCS) is one of the most common artificial sweetener widely used in many food stuff. Alpha lipoic acid (ALA) is a natural antioxidant that reduces the level of Reactive Oxygen Species (ROS). Aim of the study:  The aim of the study was to find out, whether alpha lipoic acid can mitigate the toxic effect of High Fructose Corn syrup (HFCS) on the female reproductive organ. Materials and Methods: 30 female mice were equally divided into three groups. Dose group HFCSI was administrated with 25% HFCS, while dose group HFCSII was administrated with 25%HFCS and 35mg/ kg of BW ALA for 30 days. After stipulated time, mice were sacrificed to remove ovaries for the final study. Results: Histological studies showed following defects in the ovaries of dose group CS I i.e. granulosa cells having less intact oocytes, injured primordial follicles, Cysts development, degraded oocyte and a reduced count of follicles and disarrangements of Tunica albuginea and ovarian surface epithelium. While treatment with ALA reduced the ovarian damage by improving the histopathological changes caused by HFCS.  Conclusion: The findings of this research project showed that consumption of HFCS resulted in  rise  in oxidative stress (OS) and  distorted ovarian morphology, that is histologically approved. A combined recovery with alpha Lipoic acid has also been evident and found to be  more efficient to improve reproductive pathologies, normal count of follicles, oxidative stress (OS), and histopathological score.