THE CONTENT OF METALLOPROTEINASE-2 AND METALLOPROTEINASE-9 IN THE SKIN OF RATS OF DIFFERENT AGES AFTER CLOSURE OF THE WOUND BED

Abstract

The aim: The aim of the study was to determine the content of metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9) in the skin of rats of different ages after closure of the wound bed. Materials and methods: The studies were performed on 40 white nonlinear male rats, 20 of which were 3 months old and 20 – 12 months. In each group 10 rats were control and in 10 others facelift operations were performed and cut wounds on the anterior abdominal wall were simulated. On the day of complete healing, the animals were killed, and the skin was cut in the areas of the former wound bed. In control rats, the skin was excised in the same places. The content of MMPs was determined in the skin by enzyme-linked immunosorbent assay. Results: In rats aged 3 months after re-epithelialization of the wound bed, the content of MMP-2 was 17,1% higher compared to control rats but the level of MMP-9 didn’t change. In control rats aged 12 months, the levels of MMP-2 and MMP-9 in the skin were 22,9% and 34,4% lower compared to control rats at 3 months of age. In rats 12 months of age after re-epithelialization of the wound bed, the content of MMP-2 and MMP-9 were 92,6% and 102,5% higher compared to control rats. Conclusions: We suggested that the violation of homeostasis between MMPs in rats 12 months of age disrupts wound healing and promotes the formation of pathological scars.