DISTRIBUTION OF INTEGRON III AND PHYLOGENIC CLADE AMONG MDR UROPATHOGENIC E. COLI FROM PATIENT IN AL-DIWANIYAH CITY, IRAQ

Abstract

The aim: The goal of this study is to identify Uropathogenic E. coli multidrug-resistant bacteria, as well as their genetic profile clade and correlation with dispersion integron. Materials and methods: Five hundred urine samples from UTI patients were collected in Al-Diwaniyah, Iraq. They were then investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Eosin-Methylene blue) to detect Enterobacteriacea, including Escherichia coli, the samples were identified using the Vitec 2 compact system, as well as MIC susceptibility testing to Amikacin, Levofloxacin, Cefepime, Meropenem, Nitrofurantion, and Trimethoprim-sulfamethoxazole for detect multidrug resistance isolates. Multiplex PCR was used to detect three types of integrase gene. Finally, ERIC2-PCR was used to detect the genetic profile of all isolates. Results: From 500 UTI samples, 22 isolates UPEC detected resistance to different class of antibiotic, including: 86.3% to Cefepime, 54.5% - to Trimethoprim-sulfamethoxazole, 31.8% -Levofloxacin, 18.8% - Amikacin, 18.8% - Imipenem, 0% to Nitrofurantion. Twenty of the 22 isolates had various integrase gene classes as: 54.5%, 36.3%, 72.2% for Intl I, Intl II, and Intl III, respectively; while two isolates have no integron. Conclusions: Integron III has a higher incidence and compensates other classes; isolates with triple classes are more virulent and antibiotic resistant. Their genomic profile reveals association with human urine and unique clade of relatives.