DNA SEQUENCING OF NOVEL YEAST ISOLATED FROM BLOODSTREAM INFECTIONS IN AL-NAJAF PROVINCE

Abstract

The aim: To identify of fungal isolates using PCR techniques with universal primer (ITS1-ITS4 and ITS3-ITS4). A total of 533 blood samples from cancer patients, renal failure patients and patients who underwent cardiac catheterization have been included in this study. Materials and methods: Devices and tools were used to preceding the study as shown in table (I), while biological and chemical materials are given in table (II). Results: Three groups, 44 isolates of Candida spp were isolated: 35(79.6%) isolates from cancer patients, 6(13.6%) isolates from patients with renal failure, and 3 (6.8%) isolates from patients with cardiac catheterization. These yeasts were diagnosed by conventional methods and by CHROMagar Candida medium, as well as by molecular methods to detect the regions of ITS2, ITS1, and the isolates were diagnosed as belonging to the yeast Candida spp. These isolates were also diagnosed using DNA sequencing detection technology and 12 new strains were recorded for the first time In the name of the researcher by the Japan Gene Bank. Conclusions: It was concluded that there was high susceptibility of the polymerase chain reaction technique based on ITS 1, ITS2 primers in diagnosing the types of yeasts isolated from the bloodstream with high accuracy and speed compared to other traditional methods. Therefore, the DNA sequencing method is considered one of the best rapid standard methods for the diagnosis of fungi.