REGULATION OF MICRORNA EXPRESSION LEVEL BY CHOLERETIC THERAPY IN FUNCTIONAL DISORDERS OF THE GALLBLADDER AND ОDDI’S SPHINCTER IN CHILDREN

Abstract

The alm: To study the effect of choleretic therapy on the level of microRNA expression in functional disorders of the gallbladder and Oddi’s sphincter in children. Materials and methods: Fifty patients with functional disorders of the gallbladder and Oddi’s sphincter who received standard therapy in combination with ursodeoxycholic acid, 20 patients – standard therapy without ursodeoxycholic acid, and 20 healthy children were examined. The level of expression of microRNA-378f, microRNA-4311, microRNA-4714-3p in the blood serum by the method of real-time polymerase chain reaction with reverse transcription according to the protocol TaqMan Gene Expression Assays was performed. Results: It was demonstrated that the activity profile of microRNA-4714-3p was significantly lower in those examined with functional disorders of the gallbladder and Oddi’s sphincter than in practically healthy children (p<0.05). After standard therapy combined with ursodeoxycholic acid in children with functional disorders of the gallbladder and Oddi’s sphincter, the level of expression of microRNA-378f is significantly higher than before therapy (5.23±0.70 SU and 2.02±0.57 SU respectively) (p<0.05). Against the background of standard therapy with the addition of ursodeoxycholic acid or without it, the expression profile of microRNA-4714-3p in the blood serum in children with functional disorders of the gallbladder and Oddi’s sphincter significantly decreased (1.93±0.58 SU and 1,14±0,53 SU respectively) (p<0.05). Conclusions: Ursodeoxycholic acid in children with functional disorders of the gallbladder and Oddi’s sphincter affects the activity of generation of gene regulators of the cellular mechanisms of microRNA-378f and microRNA-4714-3p.