EXPERIMENTAL CIRRHOSIS: LIVER MORPHOLOGY AND FUNCTION

Author:

Datsko Vitalij А.1,Fedoniuk Larisa Ya.1,Ivankiv Yana I.1,Kurylo Khristina I.1,Volska Аlina S.1,Malanchuk Sergij L.1,Oleshchuk Оleksandra М.1

Affiliation:

1. I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE

Abstract

The aim was to establish the morphofunctional changes of liver in the experimental cirrhosis. Materials and methods: The research was conducted on 24 white male Wistar rats. Experimental cirrhosis of the liver was simulated by oral administration of CCl4 2 g/kg 2 times weekly for three months. From the selected fragments of the liver, histological specimens were done according to the conventional method and examined by light microscopy. The activity of the enzymes of cytolysis and cholestasis (ALT, AST, alkaline phosphatase), the content of components of bile (cholesterol, bilirubin and bile acids) were determined in the serum. In the blood and liver were determined the content of the final products of metabolism of nitric oxide: NO2 - and NO3 -; in the blood – the content of ceruloplasmin, lactate, pyruvate, middle molecular-weight protein MWP1 and MWP2. In the liver – the activity of succinate dehydrogenase (SDG) and cytochrome oxidase (CHO), N-demethylase and p-hydroxylase microsomal activity. The state of the system of prooxidants-antioxidants was judged by the content in the liver of thiobarbituric acid reactive substance (TBARS), lipid hydroperoxide (LHP), concentration of sulfhydril group (GSH), catalase activities (CAT), superoxide dismutase (SOD). The content of endothelial (eNOS) and inducible (iNOS) NO synthases, the concentration of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were determined by the enzyme immunoassay. Results: Cirrhosis of the liver, which is morphologically confirmed by the presence of prominent sclerosis in the periportal zones and the formation of umbel, is accompanied by the development of cytolysis and cholestasis processes with an increase in the content of components of bile in the blood (cholesterol, bilirubin and bile acids). An increase in the content of lipoperoxidation products and disturbance of the state of the enzymatic and non-enzymatic units of the antioxidant system, decrease in the activity of mitochondrial (succinate dehydrogenase and cytochrome oxidase) enzymes have been established. The activity of the detoxification processes decreases, namely the inhibition of N-demethylase and p-hydroxylase activity of the liver microsomes, so the manifestations of endotoxicosis increase. This is accompanied with decreased content of endothelial and an increased content inducible NO synthase, a concentration of a stable metabolite of nitric oxide nitrite anion in the blood increase and a decrease in the liver. Сonclusions: Experimental CCl4 cirrhosis is characterized morphologically by sclerosis in the periportal zones and the formation of umbao. The metabolic and functional cirrhoticliver is characterized by cytolysis and cholestasis activation, inhibition of detoxication, prooxidant-antooxidant, including nitrooxidative, disbalance.

Publisher

ALUNA

Subject

General Medicine

Reference26 articles.

1. 1. Hybskaya E.U. Suchestvuet li universalnyy lrkarstvennyy preparat dlya lecheniya chronicheskoy patologii pecheni i hepatobiliarnoy sistemy? [Is there a universal drug for the treatment of chronic liver disease and hepatobiliary system?]. Liky Ukrainy. 2012; 5(161):28–30 (UA).

2. 2. Rosen H.R. Clinical practice. Chronic hepatitis C infection. N. Engl. J. Med. 2011; 364(25):2429-2438.

3. 3. Hernandes-Gea V., Friedman S.L. Pathogenesis of liver fibrosis. Annual Review of Pathology: Mechanisms of Disease. 2011; 6:425-456.

4. 4. Povero D., Busletta C., Novo E. et al. Liver fibrosis: a dynamic and potentially reversible process. Histol. Histopathol. 2010; 25:1075-1091.

5. 5. Doi K., Kurabe S., Shimazu N. Inagaki Systemic histopathology of rats with CCl4-induced hepatic cirrhosis. Laboratory animals. 1991; 25 (1):21–25.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3