Affiliation:
1. I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE
Abstract
The aim: To study pro- and antioxidant systems indicators in rats with chemically induced colon carcinogenesis on the background of the reishi mushrooms dry extract use.
Materials and methods: The study was performed on 120 white male rats. Chronic oncogenic intoxication was modeled by administering 1,2-dimethyl¬hydrazine (DMH) hydrochloride for 30 weeks (1 time per week). A dry extract from the reishi mushrooms was administered intragastrically daily at a dose of 100 mg/kg of the animal’s body weight. Blood and liver samples were taken for research monthly. The state of the pro- and antioxidant systems was studied by the content of oxidative modification of proteins products, superoxide dismutase and catalase activity, contents of reduced glutathione and ceruloplasmin.
Results: An increase in the activity of free radical oxidation processes after DMH-induced colon carcinogenesis in rats is evidenced by a decrease in the super¬oxide dismutase activity, catalase activity, content of reduced glutathione, an increase in the content of ceruloplasmin and products of oxidative modification of proteins in the blood serum and liver of animals. The effectiveness of the dry extract of reishi mushrooms and its positive effect on the state of pro- and antioxidant systems was experimentally proved.
Conclusions: The use of the dry extract of reishi mushrooms under conditions of DMH-induced colon carcinogenesis in rats led to normalization of the anti¬oxidant protection system state and the reduction of oxidative stress.
Reference21 articles.
1. 1. Herasymets I, Fira L, Mykhalkiv M et al. Activity of the inflammatory processes in rats during experimental carcinogenesis and the influence of dry extract from reishi mushrooms on them. Pharmacology Online. 2021;3:405-412.
2. 2. Kawaguchi K, Sakurai M, Yamamoto Y et al. Alteration of specific cytokine expression patterns in patients with breast cancer. Sci. Rep. 2019;9:1–12. doi:10.1038/s41598-019-39476-9.
3. 3. Herasymets I, Fira L, Medvid I et al. Research of anti-inflammatory and oncoprotective activity of the hosta lancifolia dry extract on the model of DMH-induced carcinogenesis. Pharmakeftiki. 2023;35(2):42-51.
4. 4. Konovalenko VF, Garashchenko OO, Konovalenko SV. Suchasni pidkhody do diahnostyky i likuvannya khvorykh na rak molochnoyi zalozy. Modern approaches to the diagnosis and treatment of patients with breast cancer. Onkologia. 2021;23(1):1-9. doi:10.32471/oncology.2663- 7928.t-23-1-2021-g.9320. (in Ukrainian)
5. 5. Herasymets I, Fira L, Medvid I. Application of the thick extract from maitake mushrooms for correction of metabolic disorders under the paracetamol hepatitis in rats. Pol Med J. 2021;49 (293):346–351.