DOSE DEPENDENCE OF SUBCHRONIC INFLUENCING OF ACETAMIPRID ON THE ORGANISM OF RATS FROM DATA OF MORPHOLOGICAL RESEARCHES

Abstract

The aim: To determine the dose dependence of the subchronic effect of acetamiprid on the body of rats based on the data of morphological studies of internal organs. Materials and methods: The experiment was performed on Wistar Han rats, which were orally administered acetamiprid in doses of 6, 12 and 60 mg/kg for 13 weeks. During the experiment, clinical studies were carried out, the general condition of the animals, body weight were assessed. After necropsy, the absolute and relative weight of internal organs was determined, and morphological studies of the brain, liver, kidneys, and spleen were performed with using an Olympus BX 54 light microscope and an Olympus C-5050 ZOOM camera with software Olympus DP-Soft. The research results were subjected to statistical processing using the Microsoft Excel 2010 computer program package. Results: The most pronounced manifestations of the toxic effect of acetamiprid were observed at a dose of 60 mg/kg, which indicated its hepatotoxic and nephrotoxic effects, as well as neurotoxic effects with signs of irreversible neurocyte damage. Conclusions: Morphological studies showed a dose-dependent nature and degree of expressiveness of the toxic effect of acetamiprid. According to the totality and nature of the changes revealed in the conditions of the conducted subchronic experiment on rats, no observed adverse effect level (NOAEL) was determined at the level of 12 mg/kg, no observed effect level (NOEL) – 6 mg/kg.