Action of antibacterial drugs to the biofilm form of microorganisms, which were isolated from children with community-acquired pneumonia

Abstract

Action of antibiotics to the microorganisms in their biofilm forms was detected. Strains of Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa were used in the research. Microorganisms were isoloated from children with community-acquired pneumonia. Ability of microorganisms to form biofilms was detected in 96-well plates. For detection action of antibacterial drugs to the biofilm forms of isolated microorganisms Ceftriaxon, Amikacin, Clarithromycin and Levofloxacin were chosen. Serial dilution method was used to determine susceptibility of microorganisms to the action of antibacterial drugs. All strains, which were isolated from patients with community acquired pneumonia, were able to form biofilms. Comparing optical density (OD) of S. aureus, S. pneumoniae and P. aeruginosa after action of antibiotics to the primary biofilms and OD of primary biofilms without action of antibacterial drugs revealed that in specific dilutions depth of primary biofilms with antibiotic action was thinner than depth of primary biofilms without action of antibacterial drugs. Comparing OD of S. aureus, S. pneumoniae and P. aeruginosa after action of antibiotics to the secondary biofilms and OD of secondary biofilms without action of antibacterial drugs revealed that in certain dilutions depth of secondary biofilms with antibiotic action was thicker than depth of secondary biofilms without action of antibacterial drugs. Keywords: biofilms, antibiotics, community acquired pneumonia, children.