Evaluation of behavior components in the elevated plus maze after the action of external and internal factors

Abstract

To optimize the treatment of diseases which are accompanied by disorders of the psycho-emotional sphere, it is necessary to identify differences and expose the pathogenesis of these changes appearance depending on the primary pathology. During the study, changes in the components of behavior in the elevated plus maze were studied in rats with a developed explosion-induced neurotrauma (EINT), hyper- and hypothyroidism. The experiment conduc­ted on 33 Wistar rats, of which 12 sexually mature rats were divided into control and expe­rimental (with an established EINT) group, 21 young rats were divided into 3 groups – with hyperthyroidism, hypothyroidism and a control group. All animals were kept in standard conditions of the vivarium of the DSMU, the studies were carried out in accordance with the requirements of bioethics. Analysis of the results showed that under the action of various pathological factors, different behavioral profiles are formed, which indicate the development of a trivial state during stress-like reactions after neurotrauma or with an altered hormonal state. The behavioral profile of rats after reproduction of the EINT in comparison with the control group indicates the development of stress-like conditions (statistically significant (p≤0.05) increase in upright stands by 62%, an increase in the number of bowel movements by 50%. Hyperthyroidism is characterized by sympathetic nervous system activation, as evidenced by a significant (p≤0.05) increase in both the number of measures in the open sleeves by 70% and a significant (p≤0.05) increase in the time spent in light sleeves by 51%. In hypothyroidism, there is a suppression of the emotional component of the rats behavior, as evidenced by a significant (p≤0.05) reduction of measures by 63% and time spent in light sleeves by 86%, grooming duration by 71%, hanging from the edges of open sleeves by 80% and the number of bolus defecation by 100%. Keywords: thyroid gland, antigen, staphylococcal toxoid, mast cells, experiment, rats.