Micropropagation of Pelargonium odoratissimum (L.) L’Her. through petioles and leaves-Reference-Cited by-同舟云学术

Micropropagation of Pelargonium odoratissimum (L.) L’Her. through petioles and leaves

Published:2021-03-13 Issue:2 Volume:38 Page:261-278
ISSN:2477-9407
Container-title:Revista de la Facultad de Agronomía, Universidad del Zulia
language:es
Short-container-title:RevFacAgron(LUZ)

Author:

Ebrahimzadeh Asghar¹^ORCID,Fathollahzadeh Maliheh²,Bagher Hassanpouraghdam Mohammad³^ORCID,Aazami Mavaloo Mohammad¹^ORCID

Affiliation:

1. Assitant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran

2. Former Master student, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran

3. Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran. Islamic Parliament Research Center, Tehran, Iran

Abstract

Pelargonium odoratissimum (L.) L’Her is a hard rooting plant and the common methods of propagation via stem cuttings are not successful with this species. therefore, tissue culture methods have been experienced for the mass-propagation of this high-valued species. Intact leaves, leaf segments and petiole sections derived from nodal explants in vitro were employed for the optimization of P. odoratissimum micropropagation. The treatment combinations used were MS and 1/2 MS media supplemented with 6-benzylaminopurine, BAP (1, 1.5, 2 and 4.5 mg.L-1) and 1-naphthaleneacetic acid, NAA (0.1, 1 and 1.5 mg.L-1). With leaf segments, the lowest browning incidence, the greatest callogenesis and the highest number of shoots were obtained with the media containing 1.5 mg.L-1 BAP and 1 mg.L-1 NAA. Two mg.L-1 BAP + 0.1 mg.L-1 NAA kept the same results for petiole explants. Intact leaves showed the best results for the three mentioned treatments with 1 mg.L-1 BAP + 1 mg.L-1 NAA. 0.2 mg.L-1 NAA caused the highest rooting percentage and the greatest mean data for the number and length of the roots. Rooted plantlets were transferred to the pots containing 1:1 peat-moss and perlite. Acclimatization of the plantlets was followed by 90 % of survival rate in the greenhouse. The protocol employed would be a potent one to present for the extension section.

Publisher

Revista de la Facultad de Agronomia, Universidad del Zulia

Reference24 articles.

1. Agarwal, R.K. and A.S. Ranu. 2000. Regeneration of plantlets from leaf and petiole explants of Pelargonium × hortorum. In Vitro Cell. Dev. Biol. Plant. 36:392-397.

2. Arshad, M., J. Silvestre, G. Merlina, C. Dumat, E. Pinelli and J. Kellerhoff. 2012. Thidiazuron- induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars. Plant. Cell. Tiss. Org. Cult. 108: 315-322.

3. Benazir, J.F., R. Suganthi, P. Chandrika and B. Mathithumilan. 2013. In vitro regeneration and transformation studies on Pelargonium graveolens (Geranium) an important medicinal and aromatic plant. J. Med. Plant Res. 7 (38): 2815-2822.

4. Bhuse, V.H., B.L. Lad, D.K. Patil, and V.M. Karade. 2003. Effect of time of planting, type of cutting and plant growth regulators on rooting in Pelargonium graveolens L. Herrit. Indian J. Agric. Res. 3:29-33

5. Boase, M.R., J.M. Bradley and N.K. Borst. 1998. An improved method for transformation of regal pelargonium (Pelargonium x domesticum Dubonnet) by Agrobacterium tumefaciens. Plant. Sci. 139:59–69.