Relationship of Ghrelin and Ghrelin Receptor Polymorphisms with Type 2 Diabetes

Abstract

There is compelling evidence that the gastric peptide ghrelin and its growth hormone secretagogue G protein-coupled receptor play an important role in glucose metabolism and energy homeostasis. Therefore, these genes carrying susceptibility alleles for Type 2 diabetes are considered as potential candidates. A case-control cross-sectional association study was conducted in Isparta region, including 75 Type 2 diabetes patients and 100 Turkish adult individuals, including 25 controls. Four promoters (rs26311, rs26312, rs27647, rs3755777) and an intronic (rs35683) region in the ghrelin gene and an intronic (rs509035) and a 3'UTR (rs565105) region in the ghrelin receptor are associated with type 2 diabetes by selecting single nucleotide polymorphisms Matrix Assisted Laser Desorption /Ionization was investigated using the Time of Flight Mass Spectrometer system. A significant relationship was found between type 2 diabetes and two single-nucleotide polymorphisms: rs27647 in the promoter region of the ghrelin gene and rs35683 in intron 1 (P<0.05). In conclusion, the findings suggest that ghrelin polymorphisms may predispose to disease development in the Type 2 diabetes population living in the Isparta region.