In vitro antioxidant, antiglycation, and MGO scavenging effects of Leonurus sibiricus water extract
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Published:2021-12-30
Issue:
Volume:
Page:84-95
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ISSN:2672-7277
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Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
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language:en
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Short-container-title:APJMBB
Author:
Chong Xiu Qing1, Anamalay Kirtani AP1, Nwabueze Okechukwu Patrick2, Chan Hor Kuan1
Affiliation:
1. Department of Biotechnology, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, (Taman Connaught) Cheras, 56000, Kuala Lumpur, Malaysia 2. Department of Food Science with Nutrition, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, (Taman Connaught) Cheras, 56000 Kuala Lumpur, Malaysia
Abstract
Recently, extensive research has been conducted to evaluate the inhibitory activity of different plant species on the advanced glycation end products (AGEs). L. sibiricus is a traditional herb that has been used for postpartum confinement meals in Sarawak, Malaysia and pharmacologically possess anti-hemorrhagic, antioxidant, anti-diabetic, and anti-cancer. The aim of this research was to evaluate the antioxidant, anti-AGEs, and preliminary biochemical screening of bioactive component present in L. sibiricus in water extract. Free radical scavenging activity of L. sibiricus was evaluated via DPPH, hydroxyl radical, nitric oxide, lipid peroxidation, chelating capacity, and total phenolic content was evaluated comparing with gallic acid. Inhibition of formation AGEs by L. sibiricus was evaluated using BSA-MGO, BSA-glucose and MGO scavenging assays. Flavonoids, phenols, saponins, alkaloids, phytosterol, and diterpenoids were identified in L. sibiricus extract. It also seems to inhibit early and late formation of AGE and MGO scavenging ability. L. sibiricus was able to inhibit the formation of early and late formation of AGE through the scavenging of the formation of reactive dicarbonyl intermediates and reduce the formation of methylquinoxaline adducts through the scavenge of MGO. The inhibition of AGEs formation by L. sibiricus maybe due its antioxidant property and the presence phytochemical bioactive constituents which has been previously reported to possess antioxidant and anti-AGE activity. Future research is ongoing to identify the adducts formed because of MGO scavenging by L. sibiricus using HPLC.
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Subject
Molecular Biology,Biotechnology
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