Investigation of metabolites produced by Magnaporthe oryzae during appressorium development using 1H NMR metabolomics approach
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Published:2021-09-09
Issue:
Volume:
Page:71-84
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ISSN:2672-7277
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Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
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language:en
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Short-container-title:APJMBB
Author:
Md Zain Azian1, Mohd Zainudin Nur Ain Izzati2, Ismail Intan Safinar3, Ahmad Azam Amalina4, Saad Wan Zuhainis1, Yusof Mohd Termizi1
Affiliation:
1. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 2. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 3. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 4. Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Abstract
This study was aimed to determine metabolites produced by Magnaporthe oryzae and identify metabolic changes during appressorium development. Appressorium development were induced in vitro and subjected to 1H NMR spectroscopy for metabolites production and multivariate data analysis. PCA, PLS-DA and OPLS-DA were used to profile metabolite production throughout appressorium development. There were 43 metabolites identified putatively and PCA showed differences of metabolites production between mycelium and appressorium development. Metabolites that were significantly produced (p < 0.05) during appressorium development including isocitrate, isobutyrate, lysine, glutamate, succinate, tyrosine, choline, glycerol, xylose, mannose, sucrose, tryptophan, butyrate, leucine, isoleucine, valine, ethanol, methylmalonate, threonine, lactate, alanine, arginine, 4-aminobutyrate, homoserine, glucose, mannitol and glucitol. Glycerolipid, carbohydrates and amino acids metabolisms showed to be highly involved during appressoria development. This study revealed metabolites produced by M. oryzae during appressoria development in vitro as first metabolomics data using 1H NMR approach.
Funder
Ministry of Higher Education, Malaysia
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Subject
Molecular Biology,Biotechnology
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