Analysis of normalization method for DNA microarray data-Reference-Cited by-同舟云学术

Analysis of normalization method for DNA microarray data

Published:2019-09-03 Issue: Volume: Page:30-37
ISSN:2672-7277
Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
language:en
Short-container-title:APJMBB

Author:

Baans Omar Salem¹,Jambek Asral Bahari¹,Mat Said Khairul Anuar¹

Affiliation:

1. School of Microelectronic Engineering, Universiti Malaysia Perlis, Perlis, Malaysia

Abstract

Normalization is a process of removing systematic variation that affects measured gene expression levels in the microarray experiment. The purpose is to get more accurate DNA microarray result by deleting the systematic errors that may have occurred during the making of DNA microarray Image. In this paper, five normalization methods of Global, Lowess, House-keeping, Quantile and Print-tip are discussed. The Print Tip normalization was chosen for its high accuracy (32.89 dB and its final MA graph shape was well normalized. Print tip normalization with PSNR value of 33.15dB has been chosen as a new normalization method. The results were validated using four images from the formal database for DNA microarray data. The new proposed method showed more accurate results than the existing methods in term of four parameters: MSE, PSNR, RMSE and MAE.

Funder

Kementerian Sains, Teknologi dan Inovasi

Publisher

Malaysian Society for Molecular Biology and Biotechnology

Subject

Molecular Biology,Biotechnology

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