Acute toxicity of glyphosate on various life stages of calanoid copepod, Pseudodiaptomus annandalei
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Published:2019-07-12
Issue:
Volume:
Page:24-31
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ISSN:2672-7277
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Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
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language:en
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Short-container-title:APJMBB
Author:
Lim Xue-Er1, Lai Kok-Song2, Liew Hon-Jung3, Loh Jiun-Yan1
Affiliation:
1. Faculty of Applied Sciences, UCSI University. No. 1, Jalan Menara Gading, UCSI Heights, Cheras, 56000, Kuala Lumpur, Malaysia 2. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia. 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia 3. Institute of Tropical Aquaculture (AKUATROP), Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia
Abstract
Copepods are one of the most important primary producers and biodiversity indicators. They are also highly susceptible to various toxicants. In this study, glyphosate (Roundup®), a widely used herbicide was used to investigate the toxicity effect on calanoid copepods, Pseudodiaptomus annandalei, focused on their nauplius, copepodid, and adult stages. Different concentrations of glyphosate (i.e. 0 - as control, 0.05, 0.1, 0.4, 1.6, 6.4 and 25.6 mg/L) were used to elucidate the tolerance level of P. annandalei. The survival rate of copepod was recorded at the intervals of 24, 48, 72 and 96 h after glyphosate exposure. The analysis was performed using probit test to determine the sub-lethal concentrations. Our results revealed that LC50 of the nauplius stage was recorded as 3.47, 3.02, 1.86 and 1.10 mg/L at 24, 48, 72 and 96 h, respectively. Higher LC50 values were recorded at 4.36 mg/L for 24 h, 3.09 mg/L for 48 h, 2.00 mg/L for 72 h, and 1.12 mg/L for 96 h at the copepodid stage. Generally, adult copepods showed a higher level of tolerance to glyphosate among all stages, whereby at this stage LC50 values were recorded as 11.70 mg/L for 24 h,10.23 mg/L for 48 h, 7.41 mg/L for 72 h, and 3.61 mg/L for 96 h, respectively. Our results indicated that prolong exposure time of glyphosate could increase the susceptibility of P. annandalei to the herbicide. Nauplii are the most sensitive group among all. This study showed that glyphosate could post significant eco-toxicological impact to the non-targeted organism.
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Subject
Molecular Biology,Biotechnology
Reference30 articles.
1. Abdelghani, A. A., Tchounwou, P. B., Anderson, A. C., Sujono, H., Heyer, L. R., & Monkiedje, A. 1997. Toxicity evaluation of single and chemical mixtures of roundup, Garlon‐3A, 2,4‐D, and syndets surfactant to channel catfish (Ictalurus punctatus), bluegill sunfish (Lepomis microchirus), and crawfish (Procambarus spp.). Environmental Toxicology and Water Quality 12: 237-243. 2. American Public Health Association (APHA). 2005. Standard methods for Examination of water and Wastewater, 21th Edition, APHA, Washington D C. 3. Amrhein, N., Deus, B., Gehrke, P., & Steinrucken, H. C. 1980. The site of the inhibition of the shikimate pathway by glyphosate. II. Interference of glyphosate with chorismite formation in vivo and in vitro. Plant Physiology 66: 830-834. 4. Arufe, M. I., Arellano, J., Moreno, M. J., & Sarasquete, C. 2004. Toxicity of a commercial herbicide containing terbutryn and triasulfuron to seabream (Sparus aurata L.) larvae: a comparison with the Microtox test. Ecotoxicology and Environmental Safety 59(2): 209-216. 5. Ashoka Deepananda, K. H. M., Gajamange, D., De Silva, W. A. J. P., & Wegiriya, H. C. E. 2011. Acute toxicity of a glyphosate herbicide, Roundup®, to two freshwater crustaceans. Journal of the National Science Foundation of Sri Lanka 39(2): 169–173.
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