Affiliation:
1. Department of Biology Faculty of Mathematics and Natural Sciences State University of Malang, Indonesia
Abstract
Nisaetus bartelsi is a native hawk from Java which its genetic information is not commonly understood yet. As a part of the conservation effort to maintain genetic diversity of this endangered species, this research aimed to obtain COI gene sequences from five individuals of N. bartelsi to confirm its position in the phylogenetic tree. DNA isolation from 5 N. bartelsi blood sample was performed and its COI gene sequence was amplified, sequenced, and used to reconstruct phylogenetic tree using MEGA6 with several other members of Nisaetus, Aquila, and Saggitaridae family. Furthermore, the intraspecific distance between 5 N. bartelsi samples and interspecific distance with other species were calculated using MEGA6. The result suggested that all five individuals belonged to the species Javan hawk-eagle (N. bartelsi) and were closely related to the Blyth’s hawk-eagle (Nisaetus alboniger). The DNA barcoding of the Javan hawk-eagle conducted in this study is a stepping stone to conservation efforts for the Javan hawk-eagle.
Publisher
Malaysian Society for Molecular Biology and Biotechnology
Subject
Molecular Biology,Biotechnology
Reference34 articles.
1. BirdLife International. 2016. Nisaetus bartelsi. United Kingdom: The IUCN Red List of Threatened Species.
2. Collar, N. 1997. Taxonomy and conservation: chicken and egg. Bull British Ornithologists’ Club 117: 122–136.
3. Collar, N. J., Andreev, J. V., Chan, S., Crosby, M. J., Subramanya, S., & Tobias, J. A. 2001. Threatened birds of Asia: the birdlife international red data book. Cambridge: BirdLife International.
4. Ferguson-Lees J. & Christie, D.A. 2001. Raptors of the world. London: Christopher Helm.
5. Frankham, R., Ballou, D. J., & Briscoe, D. A. 2002. Conservation genetics. Cambridge: Cambridge University Press.