Role of type IV pilin biosynthesis genes in biofilm formation of Aeromonas hydrophila-Reference-Cited by-同舟云学术

Role of type IV pilin biosynthesis genes in biofilm formation of Aeromonas hydrophila

Published:2023-03-06 Issue: Volume: Page:89-96
ISSN:2672-7277
Container-title:Asia Pacific Journal of Molecular Biology and Biotechnology
language:en
Short-container-title:APJMBB

Author:

Garg Nancy¹,Sharma Geetika¹,Saffarini Daad²,Sharda Shivani¹,Sahney Rachana¹,Shirodkar Sheetal¹

Affiliation:

1. Amity Institute of Biotechnology, Amity University Uttar Pradesh Noida Campus, Noida 201313, India

2. Department of Biological Sciences, University of Wisconsin Milwaukee, 3209 N. Maryland Ave, Milwaukee, WI 5321, USA

Abstract

Aeromonads resides in aquatic environments and infect humans and fish among other animals. This opportunistic pathogen is predicted to have several pili and fimbriae genes which may promote biofilm formation and attachment affecting the infection process. The present study compares biofilm formation and subsequent infection on MDCK cell lines using wildtype Aeromonas hydrophila and putative type IV pilin biosynthesis gene mutant generated by standard protocol. The results indicate the involvement of putative pilus biosynthesis operon AHA0686-AHA0696 in biofilm formation of Aeromonas hydrophila and infection of MDCK cells. In silico analysis of the operon predicts to contain putative type IV pili and pilin biosynthetic genes. Detailed analysis of these genes is required to evaluate the applicability of these mutant strains as potential vaccine candidates.

Publisher

Malaysian Society for Molecular Biology and Biotechnology

Subject

Molecular Biology,Biotechnology

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